Genome Engineering
The Genome Engineering Shared Resource (GESR) is dedicated to providing (at cost) state-of-the-art genome editing services for human cell lines. The GESR will initially have the capacity to make standard knockouts and knockins for your favorite gene in your favorite cell line. Ultimately GESR will provide services for making conditional alleles and epitope tagging endogenous genes, as well as carrying out whole genome knockout screens.
The goal of GESR is to utilize state-of-the-art CRISPR/Cas9-mediated gene targeting to efficiently generate desired modifications in mammalian cells in a timely manner in order to accelerate the individual researchers intellectual pursuit.
New clients should get in touch with the GESR team by emailing [email protected].
Watch a video on genome editing from the McGovern Institute.
Services
GESR cell line generation services
- Conditional knockout cell line generation
- HDR-mediated gene tagging
- Single amino acid knock-in mutation incorporation
- Multi-gene KO cell line
- Simple, single gene KO cell line
Project Support Services
- CRISPR Cas9 gRNA validation
- CRISPR Cas9 gRNA design
- HDR Donor Vector Construction
- Delivery of a bulk population of highly edited cells (Knockout)
- Genotyping Services
Custom editing available to suit all needs!
Instrumentation
- Primary Cell Engineering
- iPSC Engineering
- Base Editing
- Custom PCR Design
- Electroporation
- Fluorescent Microscopy
- Flow Cytometry
- Tyto Flow Sorting