Ryan A. Langlois, PhD

Ryan A. Langlois

1-117 MRF
689 23rd Ave SE
Minneapolis, MN 55455
United States


Immunity to influenza virus infections

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Research Summary/Interests

Immunity to influenza virus infections

Influenza A virus (IAV) represents a major global health burden. Despite yearly vaccinations the virus is able to escape seasonal immunity requiring yearly vaccination and the threat of novel pandemics loom. Therefore continued understanding of the host-pathogen interactions and protective immune responses are critical for broadly protective vaccine development. Our overall research goal is to address fundamental questions in virology and viral immunology that have been difficult to dissect using conventional approaches. We utilized host-derived microRNAs, small non-coding RNA capable of mediating silencing of mRNA, to restrict the natural tropism of IAV. This allows for previously unavailable insights into immune responses to the virus. Additionally, we generate novel reporter viruses to further define the relationship between cellular tropism and immunity as well as to determine infected cell fate. A more comprehensive understanding of virus infection requirements that dictate immunity will be critical for the design of next generation vaccines and therapeutics


  • Heaton NH*, Langlois RA*, Sachs D, Lim JK, Palese P* and tenOever BR*. 2014. Long-term survival of influenza virus infected club cells drives immunopathology. Journal of Experimental Medicine 211(9):1707-14. *contributed equally
  • Backes S, Langlois RA, Schmid S, Varble A, Shim JV, Sachs D, and tenOever BR. 2014. The mammalian response to virus infections is independent of small RNA silencing. Cell Reports 8(1):114-25.
  • Langlois RA*, Albrecht RA*, Kimble B, Sutton T, Shapiro JS, Finch C, Angel M, Chua MA, Gonzalez-Reiche AS, Xu K, Perez D, Garcia-Sastre A, tenOever BR. 2013. MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies. Nature Biotechnology 31(9):844-7. *contributed equally
  • Chua MA, Perez JT, Shmidt S, Langlois RA, tenOever BR. 2013. Influenza A Virus Utilizes Suboptimal Splicing to Coordinate the Timing of Infection. Cell Reports 3(1):23-9.
  • Pica N, Langlois RA, Krammer F, Margarine I, and Palese, P. 2012. NS1-truncated live attenuated virus vaccine provides robust protection to aged mice from viral challenge. Journal of Virology 86(19):10293-10301.
  • Langlois RA, Varble A, Chua MA, García-Sastre A, and tenOever BR .2012. Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses. PNAS Jul 24;109(30):12117-22.
  • Shapiro JS, Langlois RA, Pham AM, tenOever BR. 2012. Evidence for a cytoplasmic microprocessor of pri-miRNAs. RNA 18(7):1338-46.
  • Pham AM, Langlois RA, tenOever BR. 2012. Replication in Cells of Hematopoietic Origin Is Necessary for Dengue Virus Dissemination. PLoS Pathogens 8(1):e1002465. doi:10.1371/journal.ppat.1002465
  • Langlois RA, Shapiro JS, Pham AM, tenOever BR. 2012. in vivo delivery of cytoplasmic RNA virus-derived miRNAs. Molecular Therapy Feb;20(2):367-75.